Technology Turbidimetry - Specific proteins

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Consolidation without compromise

  • Turbidimetry is Roche’s technology for homogeneous immunoassay detection
  • Continuous development of turbidimetric technology in recent years – both in detection methods and assay design – have made turbidimetry a highly precise and sensitive detection method
  • The ability to perform specific protein analyses on an integrated clinical chemistry/immunoassay system can allow for consolidation of testing on a single platform, resulting in improved laboratory operations efficiency and significant cost savings
  • High result quality, high reliability and convenient operation all improve ease of handling - making turbidimetry attractive both for routine and emergency use

Turbidimetry setting new standards

The use of bichromatic wavelengths in spectrophotometry in conjunction with the measurement of a sample blank minimizes the effect of interference.

Consolidated, efficient and accelerated result reporting

  • Advancements in detection technology - as well as assay design - have made turbidimetry an equal detection method to nephelometry
  • Broadest specific proteins menu on a fully consolidated platform (including open channel) offering high sample throughput capability and no sample split.
  • No dedicated instrument for specific protein assays needed. 
  • Most efficient assay usage with high onboard stability and low calibration frequency
  • Options for laboratories of all sizes with standardized reagents across all platforms

Ledue, T. B., Collins, M. F., Ritchie, R. F. (2002). Development of Immunoturbidimetric Assays for Fourteen Human Serum Proteins on the Hitachi 912™. Clin Chem Lab Med. 40, 520-8
Extracts:
“We obtained excellent precision at levels corresponding to low, normal, and high physiologic concentrations of each protein.”
”The newly developed assays offer high throughput without the associated cost of a dedicated instrument for protein assays.”
“The ability to detect small changes in turbidity enables the use of relatively small sample and reagent volumes and has lead to significant cost savings in our laboratory.”

Denham, E., Mohn, B., Tucker, L., Lun, A., Cleave, P., Boswell, R.D. (2007). Evaluation of immunoturbidmetric specific protein methods using the Architect ci8200: comparison with immunonephelometry. Ann Clin Biochem. 44, 529-536
Extracts:
“Turbidimetric and nephelometric methods compared closely in analytical performance. Methods were precise and correlated well.”
“The ability to perform specific protein analyses on an integrated clinical chemistry/immunoassay system can allow for consolidation of testing on a single platform, resulting in improved laboratory operations efficiency.”

Thuillier, F., Demarquilly, C. and Szymanowicz, A. et al. (2008). Turbidimétrie ou néphélémétrie : quel choix pour les dosages de l’albumine, l’ApoA, la CRP, l’haptoglobine, l’IgM et la transthyrétine ?. Ann Biol Clin (Paris). Jan-Feb,66(1), 63-78.
Extracts:
“Le traitement par analyse de variance des moyennes pour les 6 protéines a montré un effet « automates » (p ≤ 0,01) mais pas d’effet méthode « néphélémétrie » versus « turbidimétrie».”
“Des résultats de même niveau de concordance ont été obtenus en comparant la néphélémétrie sur BN Prospec™ et la turbidimétrie sur Hitachi 912™ pour 14 protéines avec un coefficient de corrélation r supérieur à 0,97.”