Assay for the quantitative in vitro determination of von Willebrand Factor (vWF)- and glycoprotein Ib (GpIb)-dependent platelet aggregation with whole blood samples on the Multiplate® analyzer.
Ristocetin is an antibiotic known to induce thrombocytopenia and platelet agglutination. Platelets bind to von Willebrand Factor (VWF) by means of glycoprotein Ib (GPIb) receptors in the presence of ristocetin. In vitro ristocetin forms complexes with VWF which bind to GpIb and trigger platelet activation and aggregation. Reduced or absent agglutination in the presence of ristocetin may be attributed to an absence or reduction of VWF or a lack or reduction in the number of platelet GPIb receptors as may be encountered in Bernard-Soulier syndrome.1,2
- Valarche, V., Desconclois, C., Boutekedjiret, T., et al. (2011). Multiplate whole blood impedance aggregometry: a new tool for von Willebrand disease. J Thromb Haemost; 9: 1645-7.
- Jenkins, C.S.P., Phillips, D.R., Clemetson, K.J., et al. (1976). Platelet Membrane Glycoproteins Implicated in Ristocetin-Induced Aggregation. JCI. ; 57: 112-124. 3 CLSI. Platelet Function Testing by Aggregometry; Approved Guideline.